Transcriptome and metabolomic analysis to reveal the browning spot formation of ‘Huangguan’ pear

Abstract Background Browning spot (BS) disorders seriously affect the appearance quality of ‘Huangguan’ pear and cause economic losses. Many studies on BS have mainly focused physiological biochemical aspects, molecular mechanism remains unclear. Results In present study, structural characteristics with were observed via scanning electron microscopy (SEM), water loss brown spots evaluated, transcriptomic metabolomics analyses conducted to reveal underlying skin browning disorder. The results showed that occurrence was accompanied by a decrease in wax layer an increase lignified cells. Genes related biosynthesis downregulated BS, resulting BS. lignin upregulated at transcriptional level, upregulation metabolites phenylpropanoid biosynthesis. Expression calcium-related genes Cold-induced may represent key induce formation addition, demonstrated exogenous NaH 2 PO 4 ·2H O ABA treatment could inhibit incidence during harvest storage time increasing wax-related expression plant resistance, whereas transcriptomics indicated GA 3 accelerate index Conclusions this study indicate explain elucidate effects different treatments regulation